There are 17 formally recognised virus species in the genus polerovirus family luteoviridae according to the ictv classification released in. Twelve cabyv isolates were characterized by sequencing two regions of the viral genome, open. Virus identification using doubleantibody sandwich enzymelinked immunosorbent assay daselisa, followed by reverse transcriptionpolymerase chain reaction rtpcr and immunocapture icrtpcr showed that cucurbit aphid borne yellows virus cabyv was largely distributed in melon, cucumber, zucchini, squash, and watermelon crops. Cucurbit aphid borne yellows virus how is cucurbit aphid borne yellows virus abbreviated. A yellowing of buttercup squash cucurbita pepo var. Dec 30, 2015 first report of cucurbit aphid borne yellows virus in tunisia causing yellows on five cucurbitacious species. Aphis gossypii and myzus persicae transmitted the virus from naturally infected plants.
Synthesis of a fulllength infectious cdna clone of cucurbit. The genomelinked protein of potato leafroll virus is located downstream of the putative protease domain of the orf1 product. Early symptoms manifest as chlorotic spots on the lower leaves, progressing to. Subject cucurbit aphid borne yellows virus remove constraint subject. Cabyv was found to reduce significantly the yields of melon and cucumber by decreasing the number of fruit per plant but not by altering the fruit shape or quality. Genetic diversity and potential vectors and reservoirs of. Here, we present the first complete genome sequence of a cabyv isolate bl4 that was collected from pumpkin during the 2017 growing season in oklahoma.
Biological and molecular characterization of the cucurbit aphid borne yellows virus affecting cucurbits in tunisia. This virus is capable of infecting all commercial cucurbit crops. Cabyv cucurbit aphid borne yellow virus acronymattic. Laboratory of chemical life science kyoto university bioinfomatics center institute for chemical research. We do not use these to store personal information about you. Cucumber green mottle mosaic virus cgmmv of tobamovirus genus, cucumber mosaic virus cmv of cucumovirus genus, cucurbit chlorotic yellows virus ccyv of crinivirus genus, melon vein. Direct sequencing of these products indicated that 12 samples were related to cucurbit aphid borne yellows virus cabyv. In the summer of 1992 symptoms characteristic of cucurbit aphid borne yellows virus cabyv were observed on cucurbits in san joaquin, sacramento and salinas valleys in california, usa. Large populations of aphids aphis gossypii glover and severe yellowing symptoms of older leaves of cucurbits were observed in outdoor and under plastictunnel cultivation, suggesting the presence of cucurbit aphid borne yellows virus cabyv, genus polerovirus, family luteoviridae. First report of cucurbit aphid borne yellows virus infecting bitter gourd momordica charantia and teasel gourd momordica subangulata subsp. The genomelinked protein of potato leafroll virus is.
Pdf first report of cucurbit aphidborne yellows virus. Lecoq h, bourdin d, wipfscheibel c, bon m, lot h, lemaire o, herrbach e 1992 a new yellowing disease of cucurbits caused by a luteovirus, cucurbit aphid borne yellows virus. Cucurbit aphidborne yellows virus how is cucurbit aphid. Samples from 20 plants showing symptoms resembling those caused by poleroviruses were analyzed by rtpcr using the coat protein cp genespecific primers ce9ce10 of cucurbit aphid borne yellows virus cabyv juarez et al. Clover yellow vein virus cyvv is an aphid transmitted virus that can infect summer squash and was previously considered to be the severe strain of bean yellow mosaic virus. Distribution and molecular diversity of three cucurbit.
Cucurbit aphid borne yellows virus cabyv and melon aphid borne yellows virus mabyv have been found to be associated with cucurbit yellowing disease in china. Yearround ipm program for cucurbits 911 seedlings cotyledons to 4 leaves vegetative growth. How is cucurbit aphid borne yellow virus abbreviated. In protected culture, insect exclusion minimum 5052 mesh 297 micron screens can provide some control.
Cabyv is defined as cucurbit aphid borne yellow virus very rarely. Sequence analysis of a 14kb pcr product spanning part of the rnadependent rna polymerase rdrp gene, the intergenic region ir and the complete coat protein cp gene of of the 29 samples revealed the presence of three distinct polerovirus species, namely cucurbit aphid borne yellows virus cabyv, melon aphid borne yellows virus. In openfield production, use of silver reflective plastic mulches can help to repel aphids. Virus diseases are the major cause of economic losses in commercial cucurbit production in lebanon. Pdf resistance to cucurbit aphidborne yellows virus in. The genetic control of resistance to cucurbit aphidborne yellows virus cabyv. Weeds are also recognized reservoir hosts of cabyv.
Melon cantaloupe, muskmelon, and watermelon aphid pacific. Cabyv is defined as cucurbit aphidborne yellows virus plant disease somewhat frequently. Pdf cucurbit aphidborne yellows virus cabyv modifies. University of californias official guidelines for pest monitoring techniques, pesticides, and nonpesticide alternatives for managing pests in agriculture, floriculture. Zucchini yellow mosaic virus zymv is an aphid borne virus that causes yield losses and fruit quality defects in cucurbit crops. Here, we report a novel member of the genus polerovirus, zucchini aphid borne yellows virus zabyv, which was identified in zucchini grown for seed production in the xinjiang uygur autonomous region, china. First report of cucurbit aphidborne yellows virus in. Possible involvement of phloem proteins in virus transmission by aphids. Pdf cucurbit aphidborne yellows virus cabyv modifies the.
Cabyv was first described in 1992 in france 26, but 60 it was later detected infecting cucurbits in many other countries in the world 61 2,23,26,28,54. Complete genome sequences of 22 isolates of cucurbit aphidborne yellows virus cabyv, collected from melon plants showing yellowing symptom in korea during the years 202014, were determined. There are currently 17 species in this genus including the type species potato leafroll virus. Nucleotide sequence of cucurbit aphid borne yellows luteovirus. Cabyv was first described in 1992 causing yellow leaf symptoms in cucurbit. Direct evidence for the semipersistent transmission of. Our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid borne yellows virus. Cucurbit aphidborne yellows virus in egypt springerlink. Another virus, cucurbit aphid borne yellow virus cabyv, which is found in california and also causes yellowing in cucurbits, can be confused with cucurbit yellow stunting disorder virus for more. Polerovirus is a genus of viruses, in the family luteoviridae.
Pdf cucurbit aphidborne yellows virus is prevalent in. Complete genome sequences and evolutionary analysis of. Cucurbit aphidborne yellows virus in egypt, phytoparasitica. Melons with aphid resistance usually significantly delay cabyv infection. Serological and molecular detection and prevalence of. Current status of cucurbit aphid borne yellows virus in some greenhouse and open field cucumber in iran 16 results and discussion yellowing diseases have been observed worldwide in recent decades. Cucurbit aphidborne yellows virus an evidence describes the source of an annotation, e. First report of cucurbit aphidborne yellows luteovirus in.
Aphid attack all kinds of cucurbits, but they rarely affect earlyplanted muskmelons and cucumbers. Biological and molecular characterization of the cucurbit. Since then, cabyv has been found worldwide in temperate, mediterranean and subtropical climatic environments lecoq, 1999. Cucurbit aphid borne yellows virus, complete genome. Cabyv cucurbit aphidborne yellows virus plant disease. A complete genome of cucurbit aphid borne yellows virus cabyv was obtained from cucumber cucumis sativus sample 10pn from zage village, goroka, eastern highlands province, png. Cabyv stands for cucurbit aphid borne yellow virus. Yellowing and thickening of the basal leaves a and necrosis of basal leaves of older plants b was frequent. To confirm this data, all samples were tested again by rtpcr using cabyv. First report of cucurbit aphidborne yellows virus in squash. Virus diseases of cucurbits fact sheet cornell university. Cucurbit aphidborne yellows virus cabyv modifies the alighting, settling and probing behaviour of its vector aphis gossypii favouring its own spread. Cucurbit aphid borne yellows virus cabyv was first described in france in 1992 and since then has been reported in various parts of the world, including the united states. First report of cucurbit aphid borne yellows virus cabyv.
Biological and molecular characterization of a korean. Cabyv was first described in 1992 causing yellow leaf symptoms in cucurbit plants in france and belongs to genus polerovirus, family luteoviridae. Zucchini yellow mosaic virus zymv is a recently described virus disease of cucurbits, first identified in europe in 1981. Biological and molecular characterization of a korean isolate. Cucurbit aphidborne yellows virus in egypt cucurbit aphidborne yellows virus in egypt omar, ayman. Seigner 3 1 leibniz institute dsmz german collection of microorganisms and cell cultures, plant virus department, inhoffenstra.
This virus is acquired by phloemfeeding aphid vectors in a persistent manner. Incidence and management of virus diseases of cucurbits in. Cucurbit aphid borne yellows virus is prevalent in fieldgrown cucurbit crops of southeastern spain. Virus identification using rtpcr showed cucurbit aphidborne yellows virus cabyv was largely distributed in melon, oriental melon and cucumber. Comparison with other viral sequences revealed significant similarities with the orf1 products of beet western yellows virus, cucurbit aphid borne yellows virus, and beet mild yellowing virus. Zymv infects all cultivated cucurbit types including cucumber, pumpkin, rockmelon, squash, watermelon and zucchini. First report of cucurbit aphidborne yellows virus in vicia faba. First report of pepo aphidborne yellows virus in cucurbits. Cucurbit aphidborne yellows luteovirus causes a general yellowing of the older leaves, which become thick and leathery. In contrast with previously known cucurbit viruses, cabyv is a phloemlimited virus. Large populations of aphids aphis gossypii glover and severe yellowing symptoms of older leaves of cucurbits were observed in outdoor and under plastictunnel cultivation, suggesting the presence of cucurbit aphidborne yellows virus cabyv. Phloem protein partners of cucurbit aphid borne yellows virus. Cucurbit aphidborne yellows virus cabyv is an important pathogen that causes yellowing symptoms in cucurbit crops worldwide. Cabyv stands for cucurbit aphidborne yellows virus plant disease.
Molecular identification of three distinct polerovirus. Identification and characterisation of watermelon green. First report of cucurbit aphid borne yellows virus in tunisia causing yellows on five cucurbitacious species. The cotton melon aphid, one of the vectors of cabyv, is very efficient at transmitting the virus. Geographic distribution and molecular variation of isolates of three whitefly borne closteroviruses of cucurbits. This survey revealed that zucchini yellow mosaic potyvirus zymv and cucurbit aphid borne yellows polerovirus cabyv are the most common viruses of fieldgrown cucurbits.
However the disorders were first attributed to nutritional deficiencies, the use of appropriate diagnostic. First report of cucurbit aphidborne yellows virus infecting. Cotton aka melon aphids aphis gossypii one of many morphs of. Effects of cucurbit aphidborne yellows virus on the alighting behaviour of aphis gossypii under dual choice tests. The virus host range included several cucurbits including melons and. Cucurbit aphid borne yellows virus cabyv is an important pathogen that causes yellowing symptoms in cucurbit crops worldwide.
Controlling aphids with insecticides is not effective for control of viral diseases. Current status of cucurbit aphidborne yellows virus in some. Cabyv is a member of the genus polerovirus in the family luteoviridae darcy et al. Cucurbit aphid borne yellows virus listed as cabyv. First report of cucurbit aphidborne yellows virus in italy. First report of pepo aphid borne yellows virus in cucurbits in tanzania and mayotte. Plant viruses alter insect behavior to enhance their spread.
The cotton melon aphid, one of the vectors of cabyv, is very efficient at. General information about cucurbit aphid borne yellows virus cabyv0 this website uses cookies our website uses cookies to ensure that we give you the best possible online experience. Doubleantibody sandwichelisa revealed that 33 samples were infected with zucchini yellow mosaic virus and 5 with cucurbit aphid borne yellows virus cabyv. Cabyv was found to reduce significantly the yields of. Implement an insecticide spray program to control the aphid vectors. Cucurbit aphid borne yellows luteovirus causes a general yellowing of the older leaves, which become thick and leathery. Alternate hosts include crops such as lettuce latuca sativa and fodder beet beta vulgaris. Our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid borne yellows virus sabyv, infecting suakwa vegetable sponge. Cucurbit chlorotic yellows virus ccyv genus crinivirus, family closteroviridae is an emerging plant virus, and is now spreading and causing severe economic losses to cucurbit crops in many.
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